Production of anti-glucagon sera with a C-terminal fragment of pancreatic glucagon.
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چکیده
منابع مشابه
Plasma pancreatic glucagon and insulin: glucagon ratio at birth.
of delivery (14) the mean (±SE) plasma concentration of glucose was 99±7 mg/100 ml, insulin 32±6 ,uU/ml, glucagon 183±17 pg/ml. These levels were similar to those occurring in mothers who did not have an i.v. infusion, permitting pooling of the two groups for further analysis. The 't' test for paired data was used for statistical comparison of plasma concentrations of metabolite or hormones. Th...
متن کاملDevelopment of pancreatic glucagon-specific radioimmunoassay with use of synthetic human glucagon (19-29) as immunogen.
Recent reports" have described successful production of pancreatic glucagon-specific antisera with the C-terminal tryptic fragment of porcine glucagon, glucagon (19-29), as immunogen. One of the antisera, OAL-123, was efficiently used for development of a radioimmunoassay as a tool for measuring pancreatic glucagon in biologic fluids and tissue extracts2),4). Trypsin-catalyzed digestion of porc...
متن کاملIdentical biological effects of pancreatic glucagon and a purified moiety of canine gastric immunoreactive glucagon.
Because in the dog, the gastric fundus contains the largest amount of glucagon immunoreactivity (IRG), the IRG of mucosal scrapes of 105 canine stomachs was extracted by acid-ethanol and then precipitated by ether-ethanol. The IRG recovered was measured by antisera 30K, specific for glucagon and K-4023, which cross-reacts with glucagon-like immunoreactivity. Extracts of mucosa of stomach fundus...
متن کاملEvidence for transformation of glucagon-like immunoreactivity of gut into pancreatic glucagon in vivo.
The effect of gut glucagon-like immunoreactivity (GLI) devoid of pancreatic glucagon was studied in piglets. All glucagon-like peptides with an accessible C-terminal were removed from the gut extract by specific antibodies reacting with the C-terminal of the glucagon molecule. Endogenous secretion of pancreatic and gut glucagon was blocked by somatostatin infusion, and then the purified gut glu...
متن کاملA study of the physiological role of glucagon. Passive immunization with antiserum specific for pancreatic glucagon in rats.
To investigate the physiological role of glucagon in the maintenance of euglycemia, the effect of the neutralization of glucagon by an antiserum specific for glucagon on blood glucose and C-peptide immunoreactivity (CPR) levels was studied in the basal as well as in the hyperglucagonemic states caused by hypoglycemia or an intravenous infusion of arginine in rats. The antiserum employed in this...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1979
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.26.123